The aim of this study was to assess the degree of a biofilm formation by strains of P. The structure of a biofilm provides protection for bacterial cells against adverse environmental factors, including trade disinfectants and antibiotics ( 7). It is often formed on catheters and surgical implants ( 6). A biofilm is a formation of communicating microorganisms that adhere to certain surfaces and to neighboring cells and are covered with an extracellural matrix ( 6). An important adaptation of the bacteria Proteus spp., to cause infections in the urinary system, is the ability to form a biofilm. vulgaris rods have a number of virulence factors, for example, lipopolysaccharide (LPS), flagella, fimbriae, glycocalyx, and the phenomenon of adhesion and hydrophobicity of bacteria surface ( 2). mirabilis can cause purulent meningitis in newborns ( 2, 4, 5). The microorganisms of the genus Proteus also can cause skin and subcutaneous tissue infections as well as infections arising in the case of hernia plastic surgery or acute otitis media, and the microorganisms of the genus P. may be the formation of kidney stones ( 2).

One of the consequences of UTI caused by Proteus spp. mirabilis bacteria in the etiology of UTI is 7%. Urinary tract infections (UTI) are one of the most common infectious diseases, especially in a hospital environment ( 3). These microorganisms also may be present in humans and animals, mainly in the gastrointestinal tract ( 2). bacteria mainly are found in water, soil, and natural fertilizers, as well as in food products. rods is the presence of cilia, which increases their virulence, the phenomenon of swarming growth, and the ability to produce urease ( 2). penneri, all of which are pathogenic for humans ( 1). currently include four named species: Proteus mirabilis, P. Minimum biofilm eradication values of ciprofloxacin obtained in the study are close to the values of the minimal inhibition concentration (MIC).īacteria of the genus Proteus are Gram-negative rods belonging to the family Enterobacteriaceae ( 1). An MBE90 value of ciprofloxacin for isolates of P. A value of 1.0 μg/mL ciprofloxacin is MBE50 for the strains of both tested species. On the basis of the results of the absorbance of the formazan, a degree of reduction of biofilm and minimum biofilm eradication (MBE) values of MBE50 and MBE90 were determined.Īll tested strains formed a biofilm. vulgaris were evaluated by a spectrophotometric method using 0.1% 2, 3, 5-Triphenyl-tetrazolium chloride solution (TTC, AVANTORTM). strains.Ī biofilm formation of 20 strains of P. The aim of this study was to determine the value of the minimum concentration of ciprofloxacin that eradicates a biofilm of Proteus spp. An important virulence factor is their ability to form biofilms that greatly reduce the effectiveness of antibiotics in the site of infection. bacilli belong to opportunistic human pathogens, which are primarily responsible for urinary tract and wound infections.

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